Product Name | Rat CD133 Human Cells Transfected Lysate (positive control) (denatured) |
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Catalog Number | orb174679 |
Hazard Information | Non-Toxic |
Target | Rat CD133 |
Form/Appearance | 50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue |
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Storage | Store at -80°C. Aliquot to avoid repeated freezing and thawing |
Note | For research use only. |
Biological Origin | Human Cells |
Human Cells transfected lysate in which Rat CD133/PROM1/Prominin 1 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.
Application Notes | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol |
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Material Safety Data Sheet
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