Rat CD133 Human Cells Transfected Lysate (positive control) (denatured)

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Catalog Number: orb174679

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Product Overview

Product Name	Rat CD133 Human Cells Transfected Lysate (positive control) (denatured)
Catalog Number	orb174679
Hazard Information	Non-Toxic
Target	Rat CD133

Product Properties

Form/Appearance	50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue
Storage	Store at -80°C. Aliquot to avoid repeated freezing and thawing
Note	For research use only.
Biological Origin	Human Cells

Product Description

Human Cells transfected lysate in which Rat CD133/PROM1/Prominin 1 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.

Application Notes

Application Notes	Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol

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